Ujiie Yukitsugu served as a bushō and daimyō from the Sengoku to early Edo periods.

The Ujiie were an illegitimate branch of the Utsunomiya clan descended from the Fujiwara-Hokke.  Yukitsugu was born as the third son of Ujiie Naomoto (Bokuzen), who, along with Inaba Yoshimichi and Andō Morinari, was one of the Western Mino Group of Three.

In the third month of 1582, Yukitsugu deployed to Shinano Province for the Conquest of Kōshū by the Oda clan, which is the first time that he appears in historical records.

In the sixth month of 1582, Oda Nobunaga died unexpectedly in a coup d’état led by one of his senior retainers, Akechi Mitsuhide, in an event known as the Honnō Temple Incident.  Thereafter, Yukitsugu affiliated with Hashiba Hideyoshi (later known as Toyotomi Hideyoshi), participating in the Battle of Shizugatake in 1583 and the Battle of Komaki-Nagakute in 1584.  For his contributions, Yukitsugu was granted a fief of 15,000 koku in Ōmi and Ise provinces.  Beginning in 1592, during the deployments for the Bunroku Campaign on the Korean Peninsula, Yukitsugu was stationed at Nagoya Castle in Hizen Province.  In 1594, Yukitsugu shared responsibilities for the construction of Fushimi Castle for Hideyoshi.

In 1600, at the Battle of Sekigahara, Yukitsugu joined the Western Army.  Together with his older brother, Ujiie Yukihiro and Teranishi Naotsugu, he holed-up in Kuwana Castle in Ise.  After the war, he ascended Mount Kōya and, as a member of the defeated army, was removed from his position by Tokugawa Ieyasu.  The next year, he was pardoned and became a retainer of Hosokawa Tadaoki with a fief of 6,000 koku.  There are various theories regarding the timing of his demise, but it has been confirmed that based on letters from Tadaoki, at a minimum, he was living until the eighth month of 1604.  The headship of the clan was inherited by his lineal son, UJiie Mototaka, and his descendants served the Kumamoto domain in Kyūshū.