Lifespan: Eiroku 6 (1563) to 11/30 of Kanei 7 (1631)
Other Names: Kijirō (childhood)
Rank: bushō, daimyō
Title: Junior Fifth Rank (Lower), Chief of Water Provisions
Lord: Tokugawa Ieyasu → Tokugawa Hidetada → Tokugawa Iemitsu
Father: Takagi Kiyohide
Mother: Daughter of Mizuno Motouji
Siblings: Mitsuhide, Kazuyoshi, Masatsugu, Moritsugu, sister (wife of Ueda Chikatsugu), sister (wife of Tsuzuki Masatoyo), sister (wife of Yamada Shigetoshi)
Wife: Adopted daughter of Ōkubo Tadasuke
Children: Masanari, daughter (formal wife of Nagai Naokiyo)
Takagi Masatsugu served as a bushō and daimyō during the Azuchi-Momoyama and early Edo periods. Masatsugu served as the first lord of the Tannan domain in Kawachi Province.
Masatsugu was born as the third son of Takagi Kiyohide, a bushō and retainer of the Tokugawa clan.
Beginning in 1582, Masatsugu served the Tokugawa clan. He served valorously inn the Battle of Komaki-Nagakute in 1584 and in the Conquest of Odawara in 1590. He also participated in the Bunroku-Keichō Campaign on the Korean Peninsula. In 1594, Masatsugu inherited the headship of the clan with a fief of 5,000 koku. In 1600, he participated in the Second Battle of Ueda and, owing to his contributions, in 1602, his fief was increased by 2,000 koku.
In 1607, he was appointed as the ōbangashira, a position with responsibility for the collection of annual rice taxes from landowners. In 1614, during the Winter Campaign of the Siege of Ōsaka, Masatsugu defended Edo Castle, and, in the Summer Campaign the following year, served with valor during a deployment to Ōsaka. In 1617, he received an increase to his fief of 2,000 koku in Ōmi Province. In 1623, he was given a regular assignment in Ōsaka and received a further increase of 1,000 koku to his landholdings. He then moved to Tannan in Kawachi Province with a consolidated fief of 10,000 koku, becoming a daimyō and lord of the domain based in Tannan.
In the eleventh month of 1630, he died at the age of sixty-eight. He was buried at the Raikō Temple – the family temple in the Tannan domain, but there is also a grave at the Myōgen Temple. He was succeeded by his eldest son, Takagi Masanari.