Takenomata Kiyotsuna served as a bushō during the Sengoku period. He was a retainer of the Uesugi clan based in Echigo Province.
The Takenomata clan was an illegitimate branch of the Kaji clan and a member of the Sasaki clan of the Agakita Group.
Kiyotsuna was born as the son of Takenomata Kiyotada.
In 1494, Uesugi Fusayoshi inherited from his father, Uesugi Fusasada, the role as military governor of Echigo and became the seventh head of the Uesugi family. Fusayoshi received support from the deputy military governor, Nagao Yoshikage, but aiming to raise the Echigo-Uesugi family from the status of shugo daimyō to sengoku daimyō, in 1498, Fusayoshi ordered an end to a policy of the Muromachi bakufu that prohibited the military governor from exercising jurisdiction (such as to investigate crimes or levy taxes) within designated lands and manors in the province. Fusayoshi’s efforts to restrict these privileges of local landowners caused conflict with Yoshikage. In the ninth month of 1506, owing to betrayal by the Jinbō clan, Yoshikage was defeated and killed by the Ikkō-ikki in Etchū Province. He was succeeded by his son, Nagao Tamekage, who inherited the role as deputy military governor.
In 1507, Tamekage backed the adopted son of Fusayoshi, Uesugi Sadazane, and publicly declared a rebellion against Fusayoshi. Initially, Kiyotsuna joined Honjō Tokinaga, Irobe Masanaga and others to oppose Tamekage.
On 8/2 of 1507, Fusayoshi was subject to a sudden attack at his base by the forces under Sadazane and Tamekage. Fusayoshi relied upon his older brother, Akisada, to head toward the Kantō area; however, in the midst of fleeing, he was pursued by Tamekage’s soldiers who had stopped at Nōmine Castle in the Yasuzuke District of Echigo. He fled to the village of Matsunoyama, and, around 2:00 PM on 8/7, killed himself at the Amamizu Pass. The resistance by Kiyotsuna and others toward Tamekage persisted after the death of Fusayoshi.
Meanwhile, the Nakajō and Tsukiji clans from the Agakita Group affiliated with Tamekage, attacking the Irobe and others so that the rebel forces were quickly subdued. Kiyotsuna surrendered and submitted a written pledge of allegiance. Thereafter, he supported Tamekage without incident.
Later, Kiyotsuna served Uesugi Kenshin and was counted among the Seven Generals of the Uesugi along with Kakizaki Kageie, Shibata Naganori, Kaji Harutsuna, Honjō Shigenaga, Irobe Katsunaga, and Nakajō Fujisuke.