Ōtawara Tanekiyo served as a bushō during the late Muromachi and Sengoku periods.

Tanekiyo was a retainer of the Nasu clan (the Upper Nasu family) and served as the lord of Minakuchi Castle in Shimotsuke Province.

In 1514, the death of Nasu Sukechika, the head of the Upper Nasu family, gave rise to a succession struggle.  Tanekiyo, together with the Ōzeki and Kanemaru clans, backed Yamada Sukehisa (Sukechika’s natural son).  He allied with the Iono and a majority of the retainers of the Upper Nasu family, but Sukehisa was killed by Nasu Sukenaga, an adopted son of Sukechika.

After incurring an attack at his castle by Nasu Sukefusa and Ōtawara Sukekiyo (Tanekiyo’s son), Sukenaga took his own life.  Tanekiyo and others then welcomed Nasu Masasuke from the Lower Nasu family (the son of Nasu Sukefusa) as the next head and achieved a unification of the Nasu clan.

According to a compilation of family genealogies of damiyō families prepared in the Edo period, Tanekiyo died on 2/3 of Eishō 11 (1514) and Nasu Sukenaga took his own life on 6/7 of the same year so it is surmised that the dispute in the Upper Nasu family had been resolved by the time of his demise.

Takakiyo was succeeded by his second son, Sukekiyo.